| Kit Size | 100 assays |
|---|---|
| Method | Spectrophotometric at 510nm |
| Reaction Time | ~ 100min |
| Detection Limit | 0.5-100% of sample weight |
| Application Examples | Yeast preparations, mushroom preparations and other materials |
| Method Recognition | Novel method |
Megazyme β-Glucan Assay Kit (Yeast & Mushroom) , 100 assays
β-Glucan (Yeast & Mushroom) Assay Kit is suitable for the measurement and analysis of 1,3:1,6-β-glucan and α-glucan in yeast and mushroom preparations.
New, improved procedure.
Several acid and enzyme-based methods for the hydrolysis of β-glucan were compared and the best option was the method using H2SO4. For most samples, similar β-glucan values were obtained with both the optimized HCl and H2SO4 procedures. However, in the case of certain samples, specifically Ganoderma lucidum and Poria cocus, the H2SO4 procedure resulted in significantly higher values.
McCleary, B.V. & Draga, A. (2016). Measurement of β-Glucan in Mushrooms and Mycelial Products. Journal of AOAC International, Vol. 99, No. 2.
The kit components have not been altered from previous lots.
Colourimetric method for the determination of Yeast and
Mushroom β-Glucan in yeast, mushroom, foodstuffs and
other materials
Principle:
(12 M H2SO4, 0°C, 2 h)
(1) 1,3:1,6-β-Glucan + 1,3-β-glucan + α-glucan + H2O →
soluble glucan
(1 M H2SO4, 100°C, 2 h)
(2) Soluble glucan + H2O → D-glucose + laminarisaccharides (trace)
(exo-1,3-β-glucanase + β-glucosidase)
(3) Laminarisaccharides + H2O → D-glucose
(glucose oxidase)
(4) D-Glucose + H2O + O2 → D-gluconate + H2O2
(peroxidase)
(5) 2H2O2 + p-hydroxybenzoic acid + 4-aminoantipyrine →
quinoneimine + 4H2O
(amyloglucosidase)
(6) α-Glucan + H2O → D-glucose
Advantages
- Very cost effective
- All reagents stable for > 12 months after preparation
- Only enzymatic kit available
- Simple format
- Mega-Calc™ software tool is available from our website for hassle-free raw data processing
- Standard included
