| Kit Size | 100 assays |
|---|---|
| Method | Spectrophotometric at 510 nm |
| Total Assay Time | ~ 120 min (plus overnight incubation) |
| Detection Limit | 2-100% of sample weight |
| Application Examples | Plant materials, starch samples and other materials |
| Method Recognition | AOAC (Method 2002.02), AACC (Method 32-40.01) and CODEX |
Megazyme Resistant Starch Assay Kit, 100 assays
The Resistant Starch Test kit for the measurement and analysis of resistant starch in plant materials and starch samples.
Colourimetric method for the determination of Resistant Starch
in cereal products and feeds
Principle:
(α-amylase + amyloglucosidase)
(1) Non-resistant starch + H2O → D-glucose + maltose (trace)
(2) Aqueous ethanol wash + centrifugation to remove D-glucose +
maltose
(3) Dissolution of resistant starch pellet in KOH and neutralisation
(α-amylase + amyloglucosidase)
(4) Dissolved resistant starch + H2O → D-glucose
(glucose oxidase)
(5) D-Glucose + H2O + O2 → D-gluconate + H2O2
(peroxidase)
(6) 2H2O2 + p-hydroxybenzoic acid + 4-aminoantipyrine →
quinoneimine + 4H2O
Advantages
- Very cost effective
- All reagents stable for > 2 years after preparation
- Only enzymatic kit available
- Measures enzyme resistant starch
- Simple format
- Mega-Calc™ software tool is available from our website for hassle-free raw data processing
- Standard included
