| Kit Size | 100 assays |
|---|---|
| Method | Spectrophotometric at 510nm |
| Reaction Time | ~ 100min |
| Detection Limit | 0.5-100% of sample weight |
| Application Examples | Oats, barley, malt, wort, beer, food and other materials |
| Method Recognition | AOAC (Method 995.16), AACC (Method 32-23.01), EBC (Methods 3.10.1, 4.16.1 and 8.13.1), ICC (Standard No. 166), RACI (Standard Method) and CODEX (Type II Method) |
Megazyme β-Glucan Assay Kit (Mixed Linkage), 100 assays
The Beta-Glucan test kit is suitable for the measurement and analysis of Beta-Glucan (Mixed Linkage).
For the measurement of 1,3:1,4-β-D-glucan in cereal grains, milling fractions, wort, beer and other food products.
Colourimetric method for the determination of β-Glucan in
cereal grains, feed, foodstuffs, beverages and other materials
Principle:
(lichenase)
(1) β-Glucan + H2O → β-gluco-oligosaccharides
(β-glucosidase)
(2) β-Gluco-oligosaccharides + H2O → D-glucose
(glucose oxidase)
(3) D-Glucose + H2O + O2 → D-gluconate + H2O2
(peroxidase)
(4) 2H2O2 + p-hydroxybenzoic acid + 4-aminoantipyrine →
quinoneimine + 4H2O
Advantages
- Very cost effective
- All reagents stable for > 2 years as supplied
- Only enzymatic kit available
- Very specific
- Simple format
- Mega-Calc™ software tool is available from our website for hassle-free raw data processing
- Standard included
