Megazyme Protein Digestibility Assay Kit (Animal-Safe Accurate Protein Quality Score Method), 50 assays

International and local governmental regulatory bodies throughout the world have introduced standards by which the quality of protein in a food product may be judged for its completeness in delivery of the essential amino acids needed for human growth and sustenance.  These regulatory requirements not only assess the compositional presence of the essential amino acids but the digestibility of the proteins and relative efficiency of the release of the amino acids present.  The quality of a protein is judged from a regulatory standpoint as a function of the efficiency of protein digestion multiplied by the presence of essential amino acids.  This is in essence the foundation of the most widely accepted protein quality test, known as the Protein Digestibility Corrected Amino Acid Score (PDCAAS) test, required by most regulatory bodies when asserting a protein content claim on a commercial food product.

Traditionally, protein digestibility has been evaluated in vivo using rat subjects to measure the amount of protein digestion that occurs when fed a protein containing product.  While yielding suitable results this in vivo measurement process is costly and time consuming.

Additionally, this type of testing is prohibitive to food companies and individuals which uphold animal testing bans for compassionate reasons.

The Animal-Safe Accurate Protein Quality Score (ASAP-Quality Score) provided by this kit (K-PDCAAS), is an in vitro enzyme digestion method that has a very high correlation to the rat digestion model and uses the same casein standard as a completely digestible control.

Principle:

(pepsin)
(1) Proteins → proteins + peptides + amino acids

(trypsin + chymotrypsin)
(2) Proteins → proteins + peptides + amino acids

(pH 5.5, 70°C)
(3) Ninhydrin + α-amino acids → Ruhemann’s purple + RCHO + H₂O + CO₂

Advantages

• U.S. Pat. No. 9,738,920
• Control samples included

• Animal free
• High correlation to rat digestibility
• Much less expensive than rat model
• Faster turnaround time for results

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