Megazyme Papain (Carica papaya)

SKU: E-PAPN

High purity Papain (Carica papaya) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

Content: 300 mg – 10 mL
Shipping Temperature: Ambient
Storage Temperature: 4oC
Formulation: In 3.2 M ammonium sulphate
Physical Form: Suspension
Stability: > 4 years at 4oC
Enzyme Activity

Protease
EC Number

3.4.22.2
CAS Number

9001-73-4
Source

Carica papaya
Molecular Weight

23,000
Concentration

Supplied at ~ 275 U/mL
Expression

Purified from Carica papaya
Specificity

Devoid of α-amylase, β-glucanase (cellulase), endo-1,4-β-xylanase and polygalacturonanase (determined by incubating high levels of the enzyme with pure polysaccharides in highly sensitive viscometric assays).
Specific Activity

~ 9 U/mg (40oC, pH 7.0 on N, N-dimethylcasein)
Unit Definition

One Unit of activity is the amount of enzyme that will produce one µmole (181 µg) of tyrosine equivalents from N, N-dimethylcasein per minute at pH 7.0 and 40oC (colour determined using the Folin-Ciocalteu reagent).
Temperature Optima

65oC
pH Optima

7
Application examples

For research and analytical use.

Megazyme Papain (Carica papaya) Data Booklet

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