Oligo-α-1,6-Glucosidase plus β-Galactosidase

Megazyme Oligo-α-1,6-Glucosidase plus β-Galactosidase

SKU: E-BGOG

High purity Oligo-α-1,6-Glucosidase (microbial) plus β-Galactosidase (A. niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

Content: 6,000 Units Oligo-α-1,6-Glucosidase plus 6,000 Units β-galactosidase
Shipping Temperature: Ambient
Storage Temperature: 4oC
Formulation: In 3.2 M ammonium sulphate
Physical Form: Suspension
Stability: > 3 years at 4oC
Enzyme Activity

Oligo-1,6-α-Glucosidase, β-Galactosidase
EC Number

oligo-α-1,6-glucosidase: 3.2.1.10
β-galactosidase: 3.2.1.23
CAZy Family

GH13, GH35
CAS Number

oligo-α-1,6-glucosidase: 9032-15-9
β-galactosidase: 9013-11-2
Synonyms

oligo-α-1,6-glucosidase: oligosaccharide 6-alpha-glucohydrolase
β-galactosidase: beta-D-galactoside galactohydrolase
Source

oligo-α-1,6-glucosidase: Microbial
β-galactosidase: Aspergillus niger
Molecular Weight

oligo-α-1,6-glucosidase: 67,300
β-galactosidase: 125,000
Concentration

Oligo-α-1,6-glucosidase (2,000 U/mL) / β-galactosidase (2,000 U/mL)
Expression

Oligo-α-1,6-glucosidase: Recombinant from a Microbial source
β-Glucosidase: Purified from Aspergillus niger
Specificity

This enzyme mixture gives complete hydrolysis of isomaltose and lactose under the defined assay conditions, with no hydrolysis of the trisaccharide, β-D-Fruf-(2,1)-β-D-Fruf-(2,1)-β-D-Fruf.
Specific Activity

Oligo-α-1,6-glucosidase (~ 170 U/mg) / β-galactosidase (~ 170 U/mg)
Unit Definition

Oligo-α-1,6-glucosidase: One Unit of oligo-α-1,6-glucosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol (pNP) per minute from p-nitrophenyl-α-D-glucopyranoside (5 mM) in sodium acetate buffer (50 mM), pH 4.5 at 40oC.
β-galactosidase: One Unit of β-galactosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol (pNP) per minute from p-nitrophenyl-β-D-galactoside (10 mM) in sodium acetate buffer (100 mM), pH 4.5 at 40oC.
Temperature Optima

50oC
pH Optima

4.5
Application examples

Used for removal of isomaltose and lactose in the measurement of dietary fiber

Megazyme Oligo-α-1,6-Glucosidase plus β-Galactosidase Data Booklet

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