| Enzyme Activity | Isoamylase |
|---|---|
| EC Number | 3.2.1.68 |
| CAZy Family | GH13 |
| CAS Number | 9067-73-6 |
| Synonyms | isoamylase; glycogen 6-alpha-D-glucanohydrolase |
| Source | Flavobacterium odoratum |
| Molecular Weight | 83,000 |
| Concentration | Supplied at ~ 200 U/mL |
| Expression | Purified from Flavobacterium odoratum |
| Specificity | Hydrolysis of (1,6)-α-D-glucosidic branch linkages in glycogen, amylopectin and their β-limit dextrins. |
| Specific Activity | ~ 140 U/mg (40oC, pH 6.0 with 1 mM CaCl2 on oyster glycogen) |
| Unit Definition | One Unit of isoamylase activity is defined as the amount of enzyme required to release one µmole of glucose reducing sugar equivalents per minute from oyster glycogen (10 mg/mL) in MES buffer (100 mM, pH 6.0) plus 1 mM CaCl2 at 40oC. |
| Temperature Optima | 50oC |
| pH Optima | 6 |
| Application examples | Applications in carbohydrate research and in the food and feeds, and cereals industry. |
| Method recognition | AOAC Method 2000.11 and GB Standard 5009.245-2016 |
Megazyme Isoamylase (Glycogen 6-glucanohydrolase) (Flavobacterium odoratum)
Pure Isoamylase (Glycogen 6-glucanohydrolase) (Flavobacterium odoratum) for use in biochemical enzyme assays and in vitro diagnostic analysis. Isoamylase, Fructanase (E-FRMXPD) and Amyloglucosidase (E-AMGDF) are used in the enzyme hydrolysis step of two validated methods for the determination of polydextrose (a low molar mass dietary fiber) in foods: AOAC method 2000.11 and Chinese GB Standard 5009.245-2016.
| Content: | 200 Units |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 4oC |
| Formulation: | In 3.2 M ammonium sulphate |
| Physical Form: | Suspension |
| Stability: | > 4 years at 4oC |
