| Content/size | 40mL |
|---|---|
| Synonyms | glucan 1,4-alpha-glucosidase; 4-alpha-D-glucan glucohydrolase |
| Form | Stabilised liquid in 50% (v/v) glycerol |
| Stability | > 4 years at 4oC |
| Specific activity | ~ 36 U/mg (40oC, pH 4.5 on soluble starch) |
| Unit definition | One Unit of amyloglucosidase activity is defined as the amount of enzyme required to release one μmole of D-glucose reducing-sugar equivalents per minute from soluble starch at pH 4.5 and 40oC |
| Specificity | Hydrolysis of terminal α-1,4 and α-1,6 D-glucose residues successively from non-reducing ends of maltodextrins |
| Applications | Applications for the measurement of starch and dietary fiber in the food and feeds industry |
Megazyme Amyloglucosidase (Aspergillus niger), 40mL
High purity Amyloglucosidase (Aspergillus niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
EC 3.2.1.3
CAZy Family: GH15
CAS: 9032-08-0
glucan 1,4-alpha-glucosidase; 4-alpha-D-glucan glucohydrolase
From A. niger. High purity. Electrophoretically homogeneous.
Stabilised liquid in 50% (v/v) glycerol.
For use in Megazyme Total Starch and Dietary Fiber methods. Amyloglucosidase, Fructanase (E-FRMXPD) and Isoamylase (E-ISAMY) are used in the enzyme hydrolysis step of two validated methods for the determination of polydextrose (a low molar mass dietary fiber) in foods: AOAC method 2000.11 and Chinese GB Standard 5009.245-2016.
E-AMGDF-A-100ML specifically to be used with ANKOMTDF Dietary Fiber Analyzer.
Specific activity:
~ 36 U/mg (40oC, pH 4.5 on soluble starch);
~ 200 U/mL (40oC, pH 4.5, p-nitrophenyl β-maltoside);
~ 3,260 U/mL (40oC, pH 4.5, soluble starch).
Stability: > 4 years at 4oC.
