Megazyme α-Glucosidase (transglucosidase) (Aspergillus niger)

SKU: E-TRNGL

High purity α-Glucosidase (transglucosidase) (Aspergillus niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.20
CAZy Family: GH31
CAS: 9001-42-7

alpha-glucosidase; alpha-D-glucoside glucohydrolase

Highly purified. From Aspergillus niger.
In 3.2 M ammonium sulphate.
Supplied at ~ 1,000 U/mL.

Specific activity:
~ 70 U/mg (40oC, pH 4.5 on maltose);
~ 2.5 U/mg (40oC, pH 4.5 on phenyl α-glucoside).

Stability: > 4 years at 4oC.

Content/size

2,000 Units

Synonyms

alpha-glucosidase; alpha-D-glucoside glucohydrolase

Form

In 3.2 M ammonium sulphate

Stability

> 4 years at 4oC

Specific activity

~ 70 U/mg (40oC, pH 4.5 on maltose)
~ 2.5 U/mg (40oC, pH 4.5 on phenyl α-glucoside)

Unit definition

One Unit of α-Glucosidase activity is defined as the amount of enzyme required to release one μmole of glucose per minute from maltose (10 mg/mL) in sodium acetate buffer (100 mM), pH 4.5 at 40oC

Specificity

Hydrolysis of terminal, non-reducing α-1,4-linked D-glucose residues with release of D-glucose

Applications

Applications in carbohydrate and biofuels research and diagnostic and analytical procedures

Megazyme α-Glucosidase (transglucosidase) (Aspergillus niger) (E-TRNGL) Data Booklet

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