| Enzyme Activity | endo-Polygalacturonase |
|---|---|
| EC Number | 3.2.1.15 |
| CAZy Family | GH28 |
| CAS Number | 9032-75-1 |
| Synonyms | polygalacturonase; (1→4)-alpha-D-galacturonan glycanohydrolase |
| Source | Pectobacterium carotovorum |
| Concentration | Supplied at ~ 14,000 U/mL |
| Expression | Recombinant from Pectobacterium carotovorum |
| Specificity | Random hydrolysis of α-1,4-D-galactosiduronic linkages in pectate and polygalacturonans. |
| Specific Activity | ~ 540 U/mg (40oC, pH 5.5 on polygalacturonic acid) |
| Unit Definition | One Unit of endo-polygalacturonanase activity is defined as the amount of enzyme required to release one μmole of galacturonic acid from polygalacturonic acid (5 mg/mL) per min in sodium acetate buffer (100 mM), pH 5.5 at 40oC. |
| Temperature Optima | 50oC |
| pH Optima | 6 |
| Application examples | Applications in carbohydrate research and in the food industry. |
Megazyme endo-Polygalacturonanase (Pectobacterium carotovorum)
High purity endo-Polygalacturonanase (Pectobacterium carotovorum) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
| Content: | 5,000 Units |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 3.2 M ammonium sulphate |
| Physical Form: | Suspension |
| Stability: | > 4 years at 4oC |
